There are two main types: domed tortoises, which live in the cooler regions of the archipelago, and saddle-backed tortoises, which live in dry, coastal environments. ![]() ![]() Giant tortoises have thick legs and small air chambers inside their shells that help hold up their massive bodies. One of them, Chelonoidis donfaustoi, was not identified until 2015, when researchers determined that the tortoises on the island of Santa Cruz are in fact two separate species. Now, however, the scientific community generally accepts that there are 13 living species of Galápagos tortoise. The similarities among the animals were so striking that scientists long debated whether they were actually different types of the same species. By 1835, when Charles Darwin arrived for the expedition that would ultimately inspire his theory of natural selection, these tortoises had evolved into distinct yet closely related species. Scientists believe that Galápagos tortoises migrated from South America to the archipelago some two to three million years ago. However, conservation efforts have renewed hope for the survival of other giant tortoise species. As the last of his kind, Lonesome George’s death marked the extinction of the Pinta Island tortoise ( Chelonoidis abingdonii). In 2012, the death of a beloved hundred-year-old giant tortoise named Lonesome George became a global symbol of the need to protect endangered species. Many of the tortoise's subspecies are listed by the International Union for Conservation of Nature as endangered or critically endangered. (The Spanish word for tortoise is galápago.) Although the islands were once thought to be home to at least 250,000 tortoises, only about 15,000 remain in the wild today. Giant tortoises were once so abundant on the Galápagos archipelago off Ecuador that the Spanish sailors who explored the region in 1535 named the string of islands for them. ![]() They are also the world's largest tortoises, with some specimens exceeding five feet in length and reaching more than 500 pounds. These reptiles are among the longest-lived of all land vertebrates, averaging more than a hundred years. There are 13 living species of Galápagos tortoises, which are also sometimes called giant tortoises. Current Population Trend: Unknown What is a Galápagos tortoise?
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![]() Messi met with Inter Miami co-owner Beckham this spring, and that was shared publicly almost to ensure that everyone knew the sides were still talking. Still, there have been hints for months that Miami remained very much in the Messi sweepstakes. It has made the playoffs in two of its first three seasons but has yet to finish a season with a winning record or even a positive goal differential. Messi is joining a team that sits last in the Eastern Conference and just fired its coach. Inter Miami needed six years from inception to playing its first match, and its first four seasons have been less than stellar.Ĭhampions League players aim for another trophy at Nations League Final Four The Heat trailed by 21 points at the time. His move comes in a week when the NBA’s Miami Heat and NHL’s Florida Panthers are both at home in their respective title series - and the Heat, during Game 3 of the NBA Finals on Wednesday night, flashed a graphic on their scoreboard welcoming Messi to Miami. In his own words, Messi tells the definitive story of his incredible career with the Argentina national football team, providing an intimate and unprecedented look at his quest for a legacy-defining World Cup victory.”Īnd now, his story will have a Miami chapter. Apple - which is a broadcast partner of MLS - announced Tuesday that it will show a still-untitled four-part documentary series “featuring exclusive behind-the-scenes access to global superstar Lionel Messi. It took months of negotiations with MLS, the Miami ownership, Adidas and even Apple getting involved in a creative pitch to bring Messi to Miami’s pitch. “I think it’ll be great for the sport in this country, especially ahead of the 2026 World Cup. You saw how the league has kind of changed in the 15 years since he arrived, and hopefully 15 years from now we’re seeing all the growth from this addition to the league. “It’s kind of reminiscent of Beckham when he came originally. “This is obviously the biggest signing that they’ve brought in,” said Nashville defender Walker Zimmerman, a U.S. toward the end of their careers, but landing a player still no worse than near the pinnacle of his game and just a few months removed from hoisting a World Cup is simply huge. Some of the game’s biggest names - Pelé, Franz Beckenbauer, Thierry Henry and Beckham himself - have come to the U.S. might be the biggest boost ever for American soccer on the pro stage. Messi has won 10 La Liga titles and two Ligue 1 championships, seven Copa del Reys and three Club World Cups plus a Copa América and Olympic gold medal for Argentina.Īnd now he comes to MLS, and a team that is struggling - last place in the Eastern Conference, just a few days removed from the firing of coach Phil Neville (who was hand-picked by Beckham two years ago). He is a four-time Champions League winner and his 129 goals in the top club competition are second to Ronaldo’s 140. He has been to the absolute mountaintop of the game. He scored twice in last year’s World Cup final against France, a match that ended 3-3 with Argentina prevailing 4-2 on penalty kicks. ![]() In more than 17 years of representing Argentina on the international stage, he has scored 102 goals against 38 different national team opponents - 16 of those goals coming on U.S. Messi has more than 800 goals in his career for club and country, making him one of the greatest scorers in the sport’s history. At 35, Messi has nothing left to prove in the game and filled the only significant unchecked box on his resume back in December by leading Argentina to the World Cup title. The seven-time Ballon d’Or winner - the trophy given annually to the world’s best player - makes his move after two years with Paris Saint-Germain. “Although work remains to finalize a formal agreement, we look forward to welcoming one of the greatest soccer players of all time to our league.” “We are pleased that Lionel Messi has stated that he intends to join Inter Miami and Major League Soccer this summer,” read a statement from MLS. Messi’s next matches are likely to be exhibitions with Argentina against Australia on June 15 at Beijing and at Indonesia in Jakarta four days later - and then his Inter Miami debut figures to be sometime in July. ![]() “After winning the World Cup and not being able to return to Barcelona, it was my turn to go to the league of the United States to live football in another way,” Messi said. ![]() Non-traditional choices such as triangles draw attention to corners of the water features. Classic shapes like squares and rectangles are perfect for any project you have in mind. Smaller tiles are good in smaller spaces such as the area surrounding the hot tub or as part of a set-in fire pit. Beautifully detailed tiles can offer a mosaic effect that shimmers and shines all night and day. Smaller tiles are also often used when it comes to creating tile water features. A border of larger tiles set in front of much smaller tiles creates a vivid and memorable design that stands out. Large tiles can also be used in combination with other sized tiles. The larger tiles help block off the feature from the rest of the backyard. If you are planning a large water feature, large tiles are a great choice. The kind of size and shape you want will depend on the overall plan you have in mind. Using patterned tiles can also show people the location of the hot tub and the coping around the sides of the pool. It also helps people avoid the waterfall if they would rather stay on the other side of it. For example, using patterned tiles to make out a waterfall, makes it clear exactly where the waterfall begins and ends. A pattern that offers contrast from the rest of the materials help set boundaries. A pattern that uses many shades helps set the water feature apart from the surrounding area. Patterns combine different colors in a single space. Using PatternsĪnother option that appeals to many homeowners is using patterns. A few vividly colored tiles set against a neutral background help create a mood and add useful, elegant detail in any space. Think about using multiple colors in the same space. ![]() Such shades are a good choice if you love lots of color. These colors will stand out from the rest of the yard. Vivid shades of intense blue are a great way to capture the viewer’s attention and keep it there. If you want something a bit more dramatic that can serve as a focal point for the entire space, consider using darker colors. The understated colors work well with features you may already have in your backyard such as a pool or a birdbath. Lighter colors like soft brown and shades of grey are ideal for tile water features that are part of an overall look. Tiles come in nearly any color you can imagine. Start with Colorīefore doing anything else, think closely about the use of color. When thinking about a water feature, it’s a good idea to consider all the possibilities available. They come in almost any shape you would like as well as dozens of colors and patterns. Fujiwa Tiles are made from fine materials and designed to last. Tiles are durable, easy to install and incredibly varied. Tile water features are a superb way to add color, light and a sense of movement. ![]() Many homeowners find that tiles make excellent materials to create their personal water feature. The desired shape of the water feature Ĭolor, shape and material should all come together to ultimately form a harmonious whole.The first step is to think carefully about the specifics they want including: ![]() In the meantime, due to the different experimental condition, it is impossible to ensure that each group of test samples will have the same bacterial concentration, Here, the content of phosphoric acid, which is absorbed by the engineering bacteria, was expressed by the reduction of phosphate concentration (ΔCpi). In the supernatant after centrifugation, which was measured by ammonium molybdate spectrophotometry and expressed by the absorbance at the wavelength of 710nm We have determined the cell concentration (CE), which was represented by the absorbance at the wavelength of 600nm (A(600nm)), and phosphate concentration (Cpi) To confirm the function of engineering bacteria, we designed a series of experiments and finally we concluded that the engineering bacteria which was transferred anĪdditional Pst gene could absorb the phosphate more efficiently. ![]() (Just replace the solution in step 2 with deionized water)ġ2.Use the reference solution and sample solution to measure the light absorption at a wavelength of 710 nm Then add 2mL 26g/L molybdate solution and set the volume to the scale with water.ġ0.Shake volumetric flask and set aside for 15 minutes.ġ1.Prepare a reference solution in the same way. When the pressure reaches 1.1kg/cm2 and the temperature reaches 120℃, keep it for half an hour and then stop heating.ĩ.Take 2mL solution obtained in step 8 to into a 50mL volumetric flask, then add 3mL 20g/L ascorbic acid solution and wait for half a minute. (The methods to prepare them are as follows)Ģ.Take 5mL bacterial solution then centrifuge and take the sediment.ģ.Add about 5mL deionized water to the sediment and resuspend it.Ĥ.Repeat step 2&3 two or three times to wash the sediment.ĥ.Add a little bit of water (maybe 1 to 2mL) and resuspend it.Ħ.Move the solution to 15mL test tube with stopper and add deionized water to 10mL.ħ.Add 1.6mL 5% potassium peroxydisulfate solution and tie the stopper tightly with gauze and thread (or other ways) and put all the test tubes into a beaker.Ĩ.Heat the large beaker in a high-pressure steam sterilizer. Method for detecting phosphorus concentration in bacteria.ġ.Prepare 5% potassium peroxydisulfate solution, 20g/L ascorbic acid solution and 26g/L molybdate solution. (Detect the phosphate concentration every half hour, the detection method is as Then add 1mL 1mM IPTG solution and 5μL kanamycin.ħ.Grow the engineering bacterium in the condition of 37℃, 220rmp for 4 hours to 6 hours. Verify the function of engineering bacterium.ġ.First prepare the MOPS medium and LB medium.Ģ.Inoculate the engineering bacterium on LB medium till OD600=0.6~0.7.ģ.Take 5 mL suspended solution then centrifuge the bacterial solution and take the sediment.Ĥ.Add about 5mL MOPS medium to the sediment and resuspend it.ĥ.Repeat step 3&4 three times to wash the sediment to remove the residual LB medium.Ħ.Add MOPS medium to sediment up to 5mL and resuspend it. After 6 h induction, significant differenceĬan be seen, and the experimental groups with the inducer IPTG concentration greater than 1 mM all have significant expression. Significant difference in expression between the experimental group and the control group after 2h and 4h induction. His Tag is attached to the C segment of PstB protein, which is 257 AA and weighs 28 kD. Immunoblots were then visualized with chemiluminescence reagent kit. Were incubated with the appropriate secondary antibody (1:10000) at room temperature for 1 h. On the following day, wash the membrane in three washes of TBST, 10 min each. Then electrophoresed and transferred to nitrocellulose membranes (0.2 µm), which were blocked with 5% non-fat blocking grade milk and incubated with theįollowing primary antibodies overnight at 4 ℃: anti-His (1:2000). ![]() Pour out the supernatant, suspend each tube with 5mL cold PBS, and crush it with ultrasonic crusher for 5-10min.Īfter crushing, take 1mL of crushed bacterial solution from each tube, add 50ul nickel medium, and incubate it for 2h at 4 degrees with rotation.Īdd 7.5uL SDS-PAGE Loading in each tube,boil at 100 ℃ for 10min.ħ.5uL sample was loaded onto SDS-PAGE gel.Run the 5% stacking gel for 30min at 80V and run the separating gel for 1 h at 120 V. Set two variables:the concentration of IPTG and the induced time.Īfter induction, 4000 rpm, centrifugate for 15 min. ![]() PlayStation lists its own big hitters Ratchet & Clank: Rift and Horizon Forbidden West with simple "2021" dates, though it previously specified the former would arrive in the first half of the year, while the latter would turn up in 2021's second half. Definition of pragmata in the dictionary. Returnal launches on 19th March, Solar Ash in June, Little Devil Inside in July, while Stray and Ghostwire Tokyo are both due in October. Sony removed the release date windows for Square Enix's Project Athia game, CAPCOM's Pragmata game, and the GhostWire: Tokyo game from the end card of its CES 2021 video. It's a PS5 console exclusive for two years, so don't expect that on Xbox Series S/X until January 2024. Square Enix's mysterious Project Athia, in development at Luminous Productions, has been given a January 2022 release date. ![]() There's also a slightly more definitive "March" 2021 launch window for the intriguing Kena: Bridge of Spririts, instead of simply "spring". It was previously slated for launch in 2022. ![]() Hidden in the trailer's small print is word that Capcom's odd-looking astronaut and small girl-starring Pragmata will now arrive in 2023. Last night brought a new PlayStation 5 trailer broadcast during the Consumer Electronics Show (CES), and with it some changes to the release dates of various upcoming PS5 games. ![]() This is due to a phenomenon called homeostatic sleep drive, which is sometimes referred to as sleep load or sleep debt. If you stay awake for a long period of time or if you stay up late, you will fall asleep faster due to the adenosine level building up throughout the hours you've been awake. “Once you've slept for a few hours, your brain starts clearing out the adenosine, sleep drive goes down, and you may experience lighter stages of sleep and awakenings.” However, if you’re not getting the recommended amount of sleep per night but can fall asleep quickly, it could also be a sign that you’re sleep deprived. Sleep is usually deepest earlier in the night, when you first fall asleep and becomes lighter as the night goes on, Dr Bhopal adds. “The longer you stay awake, the higher the sleep drive and the faster you fall asleep.” ![]() “This is part of what contributes to ‘sleep drive’,” Dr Nishi Bhopal, a sleep specialist for SleepAdvisor and integrative psychiatrist, tells Bustle. However, in times of stress, the brain can wake up fully in these moments and the thinking, planning, problem-solving, and worrying start.”īut how does falling asleep quickly factor into this? During the day, we build up a neurotransmitter called adenosine that makes us feel sleepy. “At the end of a cycle we all briefly wake up, just a little to probably check the environment, roll over and then go back to sleep again. “We sleep in cycles of around 90-120min,” Dr Lederle told Bustle. However, good sleepers tend not to notice these brief awakenings and often report that they 'slept through.'"ĭr Katharina Lederle, a sleep and circadian specialist who works with insomniacs through her program Somnia, agrees. In fact, no-one 'sleeps through the night', that's a myth. Each cycle ends with a brief period of wakefulness and this is completely normal. The cycles tend to go like this: wake > light sleep > deep sleep > light sleep > wake. "During these cycles, we move through different phases of sleep. So, what does it mean if you fall asleep quickly then wake up after two hours? Why does it mean if I wake up after two hours of sleep?Īlison Gardiner, who is a behavioural psychologist and the founder of Sleepstation, an online sleep improvement programme, told Bustle that, if you're trying to understand why you wake up after two hours of sleep, you first need to understand "the structure of sleep." While the latter could be down to some trivial reasons - like a loud noise or forgetting to take that last-minute trip to the toilet - it could also be a symptom of a potential sleep disorder. Some find it hard to drift off, and others find that they can get to sleep easily but wake up after a few hours. But unfortunately for some, getting a good night’s sleep can be seriously elusive. That awful feeling of dragging yourself out of bed when you’ve barely slept is enough evidence. ![]() Late registration is August 8th to August10th and is $150/team.Ĭlick on the button below to go directly to the registration/payment site. Regular registration is $95 July 19th to August 7th per team Early Registration of $65 before July 18th per team 100% of the profits from this tournament go towards assisting local families to send their children to these camp experiences. The tournament was founded as a fundraiser to give scholarships for local kids and teenagers to attend life changing camps and retreats such as Spring Hill Day Camp, and Rock or 707 Retreats. All volunteers are required to obtain the following clearances prior to volunteering. ![]() Each court may have it's own referee to call fouls and violations, and tournament officials are present to help maintain a safe and fun environment. Make an account with AppGarden and upload your completed clearances. Teams are guaranteed three (3) scheduled games. There are three (3) players on the court at a time. Teams are composed of similar-aged members, with a minimum of four (4) players per team. This tournament is designed to be fun and competitive while providing plenty of playing time for all teams. 3 on 3 Basketball Tournament Hosted by Oak Pointe Church/Milford & Milford High School Basketball Programs ![]() However, ratings of 55, 50 & 45 could also result in the same average. Consider a simplified example* of an item receiving ratings of 100, 50, & 0. A high standard deviation can be legitimate, but can sometimes indicate 'gaming' is occurring. ![]() This figure is provided as the trimmed mean. Rating metrics: Outliers can be removed when calculating a mean average to dampen the effects of ratings outside the normal distribution. You can include this album in your own chart from the My Charts page! "Awaken, My Love!" collection Total Charts: The total number of charts that this album has appeared in. “Awaken, My Love!” may not be the best album of the year, but it feels like the most important-an unashamed reminder to everyone that Black Music Matters.Latest 20 charts that this album appears in: Sort ranks 2016 was a year of such social turmoil that it became necessary to remind people that Black Lives Matter. Donald Glover collects a decade’s worth of culture and condenses it into a fun package that stands fearlessly in tandem with a grave warning. “Awaken, My Love!” doesn’t try to be the kind of album you could recommend to anyone, but its worth goes beyond just a nostalgia-trip. In the half-decade since his debut album, Camp, we’ve seen Childish Gambino grow up from rapping about dick jokes to creating one of the year’s boldest artistic statements. There’s likely to be a style represented in a track that doesn’t jive with each listener, but as a whole it’s a multitalented showcase which proves dusty genres can still blow your hair back. While standout tracks like the roaring “Me and Your Mama” and the silky “Redbone” were spoiled as advance singles, there’re still some hard hits from the album-tender slow jam “Baby Boy” and album closer “Stand Tall” come to mind. ![]() Small touches from Glover and co-producer Ludwig Göransson give everything a shimmering, modern sheen that keeps things from simply sounding like a time capsule from the 70s. The album has a smooth mixture of smartly produced soul, funk, and R&B, so no tracks-perhaps with the exception of the reggae “California”-feel like out of place genre cash-ins. While Because the Internet bore a lot of shame towards modern society, the genre-versatility in “Awaken, My Love!” is a plea to hold on to what’s good, and what’s always been good about black culture.Īcross 11 tracks, Gambino adopts a soulful shriek (“Me and Your Mama”, “Riot”), a quivering falsetto (“Redbone”, “Baby Boy”), and a spiking croon (“Boogieman”, “Zombies”), all with an ease and confidence unseen in much of his previous discography. The psychedelic-soul “Riot” states things explicitly: “They tried to kill us / Love to say they feel us / But they won’t take my pride.” There’s a lot of talk of pride, used interchangeably with Gambino’s son (and future generations of black Americans), such as in the hook of “Baby Boy”: “Don’t take my baby boy / Don’t take my pride and joy”. But while much of the album is meant to be a reminder to his son to “stand tall”, Glover is in turn speaking to the black community. This is made obvious by tracks like “Me and Your Mama”, “Baby Boy”, and the sweet-sounding instrumental “The Night Me and Your Mama Met”. While the lack of rapping is sure to alienate some of Gambino’s fanbase, “Awaken, My Love!” is addressed to Glover’s son, both lyrically and stylistically. Perhaps crucial to understanding this album is another fact that Glover’s kept under wraps-earlier this year he became a father. ![]() Childish Gambino’s latest is a fearless exploration of ‘black music’ of the 70s that feels more relevant to 2016 than any album before it. Three years after the release of his last LP, the enigmatic and heavily jaded Because the Internet, Glover trades smartass rap lyrics and hazy introspection for…funkadelic soul? Coming at the end of a year full of huge and egocentric hip-hop projects, “Awaken, My Love!” isn’t just a huge left hook to the industry-it sidesteps the ‘rap game’ entirely. But as his third studio album as Childish Gambino, “Awaken, My Love!” shows, Glover keeps his ear to the ground. When he’s not starring in Hollywood films (The Martian) or hit television series (Community), or even showrunning his own series (Atlanta), the man maintains social media silence. Childish Gambino, keeps a pretty private life. Despite his flourishing careers in television and music, Donald Glover, a.k.a. If the results of this tweak were as good as he claims, you'd think he'd offer up some comparison videos, or at least FPS numbers. Last Light is likely being given treatments similar to what Crysis 2 and 3 are getting, to run on multi platform, nothing more. What this guy is suggesting is that Metro 2033 has horrible coding that needs fixing vs mere engine efficiency tweaks. That said, Last Light is said to have a lot of optimizations Metro 2033 lacks, but I think it's just that, optimizations, vs lots of bug fixes. Other than that, Metro 2033 has some pretty intense shaders that can be hard on older cards, such as the 250, but even then, it's only noticeable in Bio Mass. I played the game on my GTS 250 with max textures by doing this, and it looked and ran pretty good. Pictures - Dying Light Mod (79 photos) - Metro 2033 Last Light Anna Hentai Metro Exodus Anna Naked Naked characters metro 2033 Metro 2033 Last Light Sneujan. It would more likely be memory leaks of the typical kind, like AI, etc, if there WERE such problems.įrom what I've seen, Metro 2033 is only a resource hog with the DoF blur of Very High enabled, which you can disable in the ini. I'm also doubting there are actually 3D scripts running as he suspects. I'm kinda skeptical since some said there was an improvement and others said none. These drivers are currently only compatible with OS X Yosemite 10.10.5 and can be found here. Notice for OS X Yosemite 10.10.5 users with an Nvidia graphics card at or above the minimum specification listed below:Ĭurrently separate drivers are available to fix performance issues specifically affecting Nvidia users who are using OS X Yosemite. The legendary Ranger Mode returns - dare you play the fearsome Ranger Mode? No HUD, UI, deadlier combat and limited resources combine to create the ultimate immersive experience Two unique Play Styles : "Spartan" and "Survival" - approach the game as a slow burn Survival Horror, or tackle it with the combat skills of a Spartan Ranger in these two unique modes ![]() Rebuilt and Remastered for next-gen - with all previous DLC content included, new modes and features, and many gameplay improvement, this is the definitive version of the critically acclaimed classic that fans and newcomers alike will enjoy Immerse yourself in the Moscow Metro - witness one of the most atmospheric worlds in gaming brought to life with stunning next-gen visuals at 60FPSBrave the horrors of the Russian apocalypse - equip your gasmask and an arsenal of hand-made weaponry as you face the threat of deadly mutants, human foes, and the terrifying environment itself With the legendary Ranger Mode included to offer an extra layer of challenge and immersion, Metro: Last Light Redux offers hours of AAA gameplay for an incredible price. The former transforms Last light from a more action-oriented stealth combat experience to a fraught, slow burn fight for survival. ![]() Fans of the original game will notice new features and gameplay improvements, including new melee animations, the ability to check your watch and ammo supplies on the fly, and new full-body player animations.Īnd those who favoured the more survival-horror oriented gameplay of the cult prequel ‘Metro 2033’ will find a new way to experience the campaign thanks to the introduction of two unique Play Styles – Survival and Spartan. This definitive version also includes all previously released DLC, adding 10 hours of bonus single-player content to the huge solo campaign. Newcomers will get the chance to experience one of the finest story-driven shooters of all time an epic adventure combining gripping survival horror, exploration and tactical combat and stealth. Metro: Last Light Redux is the definitive version of the critically acclaimed ‘Metro: Last Light’, rebuilt in the latest and greatest iteration of the 4A Engine for Next Gen. As Artyom, burdened by guilt but driven by hope, you hold the key to our survival – the last light in our darkest hour… Product Overview: A civil war is stirring that could wipe humanity from the face of the earth forever. ![]() Mutants stalk the catacombs beneath the desolate surface, and hunt amidst the poisoned skies above.īut rather than stand united, the station-cities of the Metro are locked in a struggle for the ultimate power, a doomsday device from the military vaults of D6. Beneath the ruins of post-apocalyptic Moscow, in the tunnels of the Metro, the remnants of mankind are besieged by deadly threats from outside – and within. ![]() Blondes are most commonly found in Northern Europe, particularly Scandinavia and the Baltic Sea countries, where true blondism is believed to have originated to enable more efficient synthesis of vitamin D, due to northern Europe's lower levels of sunlight. This is one of the hottest teen sex tubes, you will be surely pleased with our amazing collection of teen porn clips. Malay Model | xHamster Production Sort by Relevance Type All VR Quality All FPS All Date Any dates Duration (min) 50+ Search in orientations Malay Model 25247 porn video search results for "malay model" Best Videos More Girls Chat with x Hamster Live girls now! Buy NFT & Hide Ads 00:43 SG Malay Model Sheila Sex Clip 319. 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